http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109371051-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-255 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-0275 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-255 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-112 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2018-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109371051-B |
| titleOfInvention | Extraction method suitable for obtaining large amount of high-purity salmonella outer membrane protein and application |
| abstract | The invention discloses an extraction method and application suitable for obtaining a large amount of high-purity salmonella outer membrane protein. The method comprises the following steps: (1) cloning the gene of the outer membrane protein of the salmonella into an expression vector, and then introducing the obtained recombinant expression vector into host salmonella to obtain a recombinant strain; (2) carrying out amplification culture on the recombinant strain, then centrifugally collecting thalli, and freezing and storing to obtain frozen thalli; (3) adding a protein lysate into the frozen bacteria, adding a phenylmethylsulfonyl fluoride solution, carrying out constant-temperature ultrasonic crushing, and collecting protein precipitate; (4) adding outer membrane protein extracting solution into the protein precipitate, uniformly mixing, incubating at 4 ℃, centrifuging, and collecting supernatant; (5) separating and purifying the supernatant to obtain the salmonella outer membrane protein. The method has simple steps and convenient operation, is suitable for extracting a large amount of high-purity and high-activity outer membrane protein, and can simultaneously meet the requirements of subsequent experiments on protein activity, structure, vaccine research and development and the like. |
| priorityDate | 2018-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.