http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109371035-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5b70d7db7c1981312c17ccb8fcaf9ecd |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43518 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_faa8fc0e9c80c0f200f6375ced3d17bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf5e86f35a0423a783f87c82bbdaeea8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e6aa190158c142bf6a237456b912db6 |
| publicationDate | 2019-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109371035-A |
| titleOfInvention | A kind of gene and preparation method of Piriformis silk protein |
| abstract | The present invention relates to a gene and a preparation method of the piriform gland silk protein of Orchard vulgaris, and the gene sequence is shown in SEQ ID NO.1. Amplification method: Degenerate PCR was used to amplify the NT sequence of part of the piriform protein gene, and anchored PCR was used to complete the NT sequence. A pair of primers were designed according to the NT sequence obtained by sequencing and the CT sequence obtained before. It is used to amplify the full-length PySp1 gene by PCR, blunt-end cloning of the amplified fragments, and extract plasmids from the positive clones containing the full-length PySp1 gene, and perform full-length sequencing to obtain the nucleotide sequence of the gene. . The present invention obtains for the first time the gene encoding the piriform gland silk protein of the large-bellied orchard including the complete NT end, the CT end and the intermediate repeat region, which lays a foundation for further research on the structure of the piriform gland silk protein and the artificial preparation of spider silk fibers. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114269398-A |
| priorityDate | 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 319.