| abstract |
The invention discloses a serum-free medium for large-scale culture of human umbilical cord mesenchymal stem cells. The main components include amino acids, inorganic salt components, growth factors and albumin components. The serum-free medium for large-scale culture of human umbilical cord mesenchymal stem cells of the present invention does not contain any serum components and has clear components, overcomes the danger of foreign protein contamination and pathogenic microorganisms, and solves the biological safety problem of clinical application of MSCs . Using the serum-free medium of the present invention, human umbilical cord mesenchymal stem cells can be cultured on a large scale in vitro, and high-quantity, high-purity, safe and high-quality umbilical cord mesenchymal stem cells can be obtained, and at the same time, the cell survival rate can be improved, and the characteristics of stem cells can be maintained. The invention can solve the problem of insufficient cell number, and the cell number, survival rate and immune phenotype of each batch are more stable, and meet the requirements of relevant indicators. |