http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109358134-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-067 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
filingDate | 2018-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109358134-B |
titleOfInvention | A kind of pretreatment method and quantitative detection method of endogenous phosphate sugar compounds in plant samples |
abstract | The invention discloses a method for analyzing a variety of phosphate sugar compounds including endogenous trehalose-6-phosphate in a plant sample. In this method, endogenous phosphate sugars are first extracted with a solvent, and then the hydrophobic impurities in the extract are adsorbed and removed by a reverse-phase SPE cartridge, and then the diazo group in the derivatization reagent and the phosphate group shared by the phosphate sugar compounds are used. The derivatization reaction of the clusters, combined with liquid chromatography-mass spectrometry, realized the separation and detection of endogenous sugar phosphate isomers in plant samples. The method is simple, accurate, and high-throughput, which can not only realize the baseline separation of phosphate sugar isomers on a hydrophilic chromatographic column, but also ensure high detection sensitivity. Separation and detection of sexual phosphate sugars. |
priorityDate | 2018-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.