http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109355404-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2018-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109355404-B |
| titleOfInvention | Primer, kit and detection method for isothermal detection of listeria monocytogenes based on polymerase helix reaction |
| abstract | The invention discloses a primer, a kit and a detection method for constant-temperature detection of Listeria monocytogenes based on polymerase helix reaction. The primer comprises detection primers Ft and Bt; wherein the nucleotide sequence of the detection primer Ft is shown as SEQ ID NO. 1; the nucleotide sequence of the detection primer Bt is shown as SEQ ID NO. 2. The invention also provides a kit for detecting listeria monocytogenes at constant temperature based on polymerase helix reaction, which comprises the primer, Bst DNA polymerase, a mixed solution of calcein and manganese chloride and the like. The primer or the kit can be used for detecting the polymerase helix reaction of the listeria monocytogenes, can not cause time loss due to temperature change, has short time consumption, simple and convenient reaction process, short detection period and strong specificity, and can observe the detection result by naked eyes. The invention has important significance for the development of amplification of a novel constant-temperature amplification technology and the field detection of microorganisms. |
| priorityDate | 2018-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.