http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109355355-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_88d919c2b2eae29f8938a3af1608da26 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2018-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbd1c5021d51d2ba45a021fee79d3ad1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d0a103a8fb9cdeb6d0079d5b0e0664d |
| publicationDate | 2019-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109355355-A |
| titleOfInvention | A kit, preparation method and application for target DNA enrichment |
| abstract | The present application discloses a kit, preparation method and application for target DNA enrichment. The kit of the present application includes a double-stranded DNA probe and a homologous recombinase; the double-stranded DNA probe is labeled with biotin; the homologous recombinase is used to bind to the single-stranded DNA of the sample to be tested, and promote the double-stranded DNA The probe binds to homologous single-stranded DNA in the sample to be tested. The kit used in this application for target DNA enrichment adopts double-stranded DNA probes and utilizes the target recognition function of homologous recombinase to target and enrich the single-stranded target DNA in the sample to be tested; and based on homologous recombinase The double-stranded DNA probe homology single-stranded target DNA recognition and enrichment has high efficiency, good uniformity and stability, and is especially suitable for the enrichment of multi-target DNA or target DNA with low copy number. In the kit of the present application, the preparation method of the double-stranded DNA probe of the core component is simple, easy to operate, and low in cost. |
| priorityDate | 2018-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.