http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109355354-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aa5d09c18e643544e06251743a8c0a82 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2018-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_386762ec4edf430cbc12d2101c269ffb |
| publicationDate | 2019-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109355354-A |
| titleOfInvention | Methods and compositions for the production of DNA libraries directly from FFPE tissue samples |
| abstract | Methods and compositions are disclosed for preparing DNA libraries from slide-mounted FFPE tissue samples for downstream next-generation sequencing. In one embodiment, a method of preparing a DNA library is disclosed. The method may include applying droplets of the reagent mixture to a slide-mounted FFPE tissue sample. The reagent mixture may comprise one or more buffers, cofactors, non-ionic surfactants, glycerol solution, gelatin solution, dNTPs, DNA polymerase, and a primer pool comprising a plurality of forward and reverse primers. The method may also include stirring the droplets in a circular motion on the glass slide while scraping the portion of the FFPE tissue sample mounted on the glass slide to generate a reaction mixture. The method may further comprise aspirating the reaction mixture from the slide directly into a pipette tip and dispensing the reaction mixture into reaction vessels for further amplification. |
| priorityDate | 2017-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.