http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109355326-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-12 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-12 |
| filingDate | 2018-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109355326-B |
| titleOfInvention | Method for improving L-methionine yield |
| abstract | The invention discloses a method for improving the yield of L-methionine, belonging to the technical field of fermentation. The method comprises the following steps: recombinant Escherichia coli E.coli W3110 delta IJAHFEBC/pAm is taken as a production strain, inoculated in a fermentation medium and cultured to OD 600 When the concentration is 0.6-1.0, adding an inducer IPTG (isopropyl-beta-D-thiogalactoside), continuously culturing until the fermentation is finished to obtain fermentation liquor containing L-methionine, and separating and purifying the fermentation liquor to obtain the L-methionine; the fermentation medium comprises a basic medium and a cofactor, and the cofactor comprises VB 12 . In the fermentation culture process, the concentration of a carbon source of a culture medium is optimized, precursor amino acid and cofactors are added, and the fermentation condition is optimized, so that the fermentation yield of the L-methionine is improved, and the fermentation yield of the L-methionine can be obviously improved by simultaneously adding a plurality of cofactors. |
| priorityDate | 2018-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.