http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109355257-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0667 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0668 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2018-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109355257-B |
| titleOfInvention | Mixed culture method of mesenchymal stem cells from different tissue sources |
| abstract | The mixed culture method of the mesenchymal stem cells with different tissue sources comprises the following steps: separating and purifying mesenchymal stem cells from adipose tissues, bone marrow tissues and umbilical cord tissues of mammals, marking the mesenchymal stem cells separated and purified from the adipose tissues as first mesenchymal stem cells, marking the mesenchymal stem cells separated and purified from the bone marrow tissues as second mesenchymal stem cells, and marking the mesenchymal stem cells separated and purified from the umbilical cord tissues as third mesenchymal stem cells; mixing the first mesenchymal stem cell, the second mesenchymal stem cell and the third mesenchymal stem cell according to the ratio of 2:3:1, and carrying out mixed culture. When mixed culture is adopted, various cell factors secreted by mesenchymal stem cells of different tissue sources can mutually promote proliferation, and meanwhile, the added autologous umbilical cord blood serum enables the proliferation to be faster and the cell consistency to be better, so that the problems of inconsistent proliferation, slow proliferation, less passage and the need of culture media of various specifications are solved. |
| priorityDate | 2018-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.