| abstract |
The present invention relates to plant genetics and breeding technical fields, more particularly to the construction method of activation label carrier and bluish dogbane seed mutant library.Compared with prior art, the present invention leads to the foundation of the selection of explant, genetic transformation and regenerating system, can obtain large-scale bluish dogbane mutant seeds group rapidly；Sequence label (4 concatenated 35s enhancers) activation label is activated to be randomly distributed in entire bluish dogbane genome in the mutagenized populations, enhance the expression of its contiguous gene, induction forms mutant, and the mutant generated is dominant mutation, avoids the lethal effect generated by mutation of function forfeiture type. |