http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109320572-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H17-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00 |
filingDate | 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109320572-B |
titleOfInvention | Method for extracting flavonoid compounds from camellia reticulata |
abstract | The invention provides a method for extracting flavonoid compounds from camellia reticulata. The method comprises leaching Yunnan camellia tea cake to obtain extractive solution, and distilling under reduced pressure to obtain concentrated solution; adding into macroporous resin chromatographic column, gradient eluting with water, 20% ethanol, 50% ethanol and 80% ethanol, and collecting eluate to obtain crude fraction; adding the crude fraction into RP-C18 filler chromatographic column, performing gradient elution with acetonitrile-formic acid aqueous solution system, concentrating under reduced pressure, and drying to obtain crude isolate; dissolving the crude isolate with solvent, introducing sample for gradient elution, concentrating, and lyophilizing to obtain pre-isolate by using C18 chromatographic column and methanol-formic acid water solution system as mobile phase; using a C18 chromatographic column and using acetonitrile-formic acid aqueous solution as a mobile phase, dissolving a pre-separation substance by using a solvent, then injecting a sample for isocratic elution, concentrating and freeze-drying to obtain the target compound. The method provided by the application separates the target compound from the camellia plant for the first time, and the extraction purity is high. |
priorityDate | 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.