http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109320572-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H17-07
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00
filingDate 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109320572-B
titleOfInvention Method for extracting flavonoid compounds from camellia reticulata
abstract The invention provides a method for extracting flavonoid compounds from camellia reticulata. The method comprises leaching Yunnan camellia tea cake to obtain extractive solution, and distilling under reduced pressure to obtain concentrated solution; adding into macroporous resin chromatographic column, gradient eluting with water, 20% ethanol, 50% ethanol and 80% ethanol, and collecting eluate to obtain crude fraction; adding the crude fraction into RP-C18 filler chromatographic column, performing gradient elution with acetonitrile-formic acid aqueous solution system, concentrating under reduced pressure, and drying to obtain crude isolate; dissolving the crude isolate with solvent, introducing sample for gradient elution, concentrating, and lyophilizing to obtain pre-isolate by using C18 chromatographic column and methanol-formic acid water solution system as mobile phase; using a C18 chromatographic column and using acetonitrile-formic acid aqueous solution as a mobile phase, dissolving a pre-separation substance by using a solvent, then injecting a sample for isocratic elution, concentrating and freeze-drying to obtain the target compound. The method provided by the application separates the target compound from the camellia plant for the first time, and the extraction purity is high.
priorityDate 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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