http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109320445-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1029 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D209-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D209-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D209-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09K11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D209-08 |
| filingDate | 2018-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109320445-B |
| titleOfInvention | Synthesis method of Cy-series fluorescein and application of Cy-series fluorescein in DNA sequencing |
| abstract | The invention discloses a synthesis method of Cy series fluorescein and application thereof in DNA sequencing, wherein the method comprises the following steps: A. reacting the reactants With the reactant YCH 2 (CH 2 ) 4 COOH or YR are refluxed to obtain a reaction intermediate And B. subjecting the product obtained in step A to And reaction to produce Then directly adding into Generating a target product by a one-pot method; in each structural formula, Y is Cl, Br and I; r ═ Me, ethyl, propyl; x ═ O, CH (CH) 3 ) 2 ；R 1 ‑R 4 Optionally H or SO 3 ‑ Or R 1 ,R 2 Together with the benzene ring to form a naphthalene ring and derivatives, or R 3 ,R 4 Together with the benzene ring, form a naphthalene ring and a derivative; n is 1,3, 5. The method obtains the required fluorescein with the asymmetric structure by a one-pot method, has good reaction selectivity, is easy to separate and purify, has simple required experimental conditions, has conventional chemical reaction in the synthetic process, and can be popularized and used in large scale. |
| priorityDate | 2018-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.