http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109312396-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8adaddc602ad7cb7f9e2d550681bb36d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502792 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1075 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2017-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54faa3f9ca3ffe0a9443abc8163f136e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bcd9a697b38c08ac11b1ac3497461a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c0909d014750da73db1c680e850f9b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c51ee535a145da7cae44ce40d8eae3ee |
| publicationDate | 2019-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109312396-A |
| titleOfInvention | Methods and systems for constructing standardized nucleic acid libraries |
| abstract | The present disclosure generally relates to nucleic acid amplification systems and methods suitable for use in the construction of nucleic acid samples, including the construction of standardized nucleic acid libraries. In some embodiments, the method includes providing one or more input nucleic acid samples such that each of the input nucleic acid samples (eg, input library) is combined with a primer that includes a first amplification or normalization primer and a second amplification or normalization primer The reaction mixture is contacted, wherein the first amplification or normalization primer is immobilized on the solid support and the second amplification or normalization primer is in solution, and in such a manner that substantially all of the first amplification or normalization primer is incorporated into the amplification Amplify the input nucleic acid sample under conditions in which the product is amplified. Also provided are systems and droplet actuator devices configured to perform the methods disclosed herein. Also provided are compositions comprising nucleic acid samples and libraries, preferably standardized libraries, prepared according to the disclosed methods and systems. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112080806-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022021163-A1 |
| priorityDate | 2016-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 297.