abstract |
Provided herein are methods and compositions for preparing guide nucleic acids (gNAs), nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding collections of gNAs from nucleic acids of any source. Also provided herein are methods and compositions for using the resulting gNAs, nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding collections of gNAs in various applications. |