http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109306338-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-74 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0653 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
filingDate | 2018-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109306338-B |
titleOfInvention | Method for inducing differentiation of adipose-derived stem cells in vitro by using microporous coating |
abstract | The invention discloses a method for inducing differentiation of adipose-derived stem cells in vitro by using a microporous coating, belonging to the field of biomolecules. The method comprises the following steps: (1) preparing a polyelectrolyte coating by a layer-by-layer self-assembly method; (2) carrying out acid treatment on the coating, and then carrying out freeze drying to form a communicated microporous structure; (3) loading a plurality of bioactive molecules into the coating of the microporous structure; (4) the coating loaded with bioactive molecules realizes the healing of micropores in a saturated humidity environment; (5) the coating loaded with bioactive molecules can regulate and control the release of various bioactive molecules, and is maintained in a proper concentration range for more than 16 days; (6) the coating was placed in a culture plate for cell culture. The controlled-release bioactive molecule can keep good bioactivity under physiological conditions, reduce the replacement frequency of cell culture solution and reduce the cost of cell culture. |
priorityDate | 2018-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.