http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109295227-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f11f80ad31a5ddd8583124bb892d9787 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 |
| filingDate | 2018-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_490ebde7a920b16d580a1d2f4b1b75da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_767b2823a4dced3af2a4d5d8266cea46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48a6ade9121730295a96cca89a371d8e |
| publicationDate | 2019-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109295227-A |
| titleOfInvention | The optimization method and testing product of EGFR gene T790M mutation digital pcr detection architecture |
| abstract | The present invention relates to a kind of optimization methods of EGFR gene T790M mutation digital pcr detection architecture, and detection architecture includes upstream primer, downstream primer, wild-type probe, saltant type probe, wild-type template and saltant type template；Wild-type template is the normal human gDNA after digestion, and saltant type template is the mutant plasmid of the insertion EGFR gene T790M mutant fragments after digestion；Saltant type template and wild-type template are configured to standard items in certain copy number ratio, digital pcr is used to be reacted using middle frequency of mutation standard items as template, data statistics figure is made according to response data, selectes the fluorescence area of wild type and the fluorescence area of saltant type.It uses digital pcr to be reacted by template of wild-type template, determines background threshold.Testing product accuracy after the optimization method optimization of EGFR gene T790M mutation digital pcr detection architecture through the invention is higher. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114277142-A |
| priorityDate | 2018-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 561.