http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109294936-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y110-03002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C02F3-342 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0061 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C02F3-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
filingDate | 2018-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109294936-B |
titleOfInvention | Heterologous recombinant Pichia pastoris engineering bacterium GS115-pPIC 9K-LacWLF and application thereof |
abstract | The invention discloses a heterologous recombinant pichia pastoris engineering bacterium GS115-pPIC 9K-LacWLF, which carries a target gene derived from a Bacillus pumilus (Bacillus pumilus) CotA laccase superior mutant GWLF; and recombining the optimized GWLF laccase gene into a pichia pastoris GS115 genome to obtain the engineering bacterium. The recombinant pichia pastoris positive strain can be used for preparing the recombinant GWLF laccase through methanol induction culture, fermentation condition optimization and 5L fermentation tank high-density fermentation. |
priorityDate | 2018-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 378.