abstract |
One plant of anti-diclazuril monoclonal antibody specific hybridoma cell strain and its application, belong to food safety technical field of immunological detection.One plant of anti-diclazuril monoclonal antibody specific hybridoma cell strain SS0711, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC No.14689.Anti- diclazuril monoclonal antibody specific is that the anti-diclazuril monoclonal antibody specific hybridoma cell strain SS0711 secretion of CGMCC No.14689 generates by the deposit number.It is applied to the remaining analysis detection of diclazuril in food safety detection.The anti-diclazuril cell strain of monoclonal antibody that the present invention obtains has preferable detection sensitivity and affinity, the IC of monoclonal antibody of the present invention to diclazuril 50 =0.49 ng/mL, the detection range of linearity are 0.16-1.55 ng/mL, and lowest detection limit value (LOD) is 0.08 ng/mL. |