http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109270144-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3275 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2018-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109270144-B |
| titleOfInvention | Method for detecting 5-hydroxymethylcytosine based on non-labeled and non-immobilized electrochemical magnetic biosensor |
| abstract | The invention belongs to the technical field of biology, and particularly relates to a method for detecting 5-hydroxymethylcytosine by using a non-label non-immobilized electrochemical magnetic biosensor for non-disease diagnosis. Quantitative analysis of 5-hydroxymethylcytosine using an electrochemical magnetic biosensor with screen printed carbon electrodes based on a dual signal amplification strategy of terminal transferase and ru (iii) redox cycling. The method comprises the following specific steps: biotinylation of the 5-hydroxymethylcytosine site in the DNA strand; enrichment of biotinylated 5-hydroxymethylcytosine DNA strands with streptavidin-coated magnetic beads; terminal transferase catalyzes the extension of the polymerized biotinylated 5-hydroxymethylcytosine DNA strand; adding Ru (NH) 3 ) 6 3+ /Fe(CN) 6 3‑ The system produces an enhanced electrocatalytic signal; and (3) carrying out quantitative analysis on the electrochemical magnetic biosensor. The lowest detection limit of the method can reach 9.06 fM. |
| priorityDate | 2018-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.