http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109266763-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-385 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2018-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109266763-B |
titleOfInvention | Method for rapidly detecting pseudomonas aeruginosa virulent strain, detection kit and application |
abstract | The invention relates to a method for rapidly detecting a pseudomonas aeruginosa virulent strain, a detection kit and application, wherein the detection method adopts a triple PCR method, simultaneously detects pseudomonas aeruginosa exoT, exoS and toxA virulent genes, and if gene fragments of exoT, exoS and toxA can be simultaneously amplified, the detected pseudomonas aeruginosa is the virulent strain; if the gene segments of exoT, exoS and toxA are not amplified simultaneously, the detected pseudomonas aeruginosa is not a virulent strain. The triple PCR method adopts three pairs of specific primers which can respectively amplify an exoT gene segment, an exoS gene segment and a toxA gene segment. The invention also provides a PCR primer group for rapidly detecting the pseudomonas aeruginosa velogenic strain, a kit containing the primer group for rapidly detecting the pseudomonas aeruginosa velogenic strain, and application of the kit in detecting the marten pseudomonas aeruginosa. The invention has the advantages of convenient and fast detection, strong specificity, high sensitivity and the like, and can quickly and accurately realize the detection of the pyocyaneus virulent strain. |
priorityDate | 2018-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.