http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109266728-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_489aa2cd1ae60fe92ef599b7516b721d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2018-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b3331028fc870d5d63cd243a67e8637 |
| publicationDate | 2019-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109266728-A |
| titleOfInvention | Method for localizing damage and modification sites on genomic DNA |
| abstract | The invention provides a method for locating damage and modification sites on genomic DNA, firstly transforming the damage or modification site of the DNA sample into a cleavage site; then adding thionucleotide and DNA polymerase I for nick translation; The sample obtained after the termination of the reaction is added with a phosphorylthiolated DNA sensitive nuclease digestion template; finally, the obtained phosphorylsulfation modified DNA is sequenced, and the 5' end of the obtained sequence is the original damage or modification site. The method provided by the invention fills the gap that the prior art at home and abroad cannot accurately locate the damage site on the genomic DNA, and can realize the quantitative and localized analysis of the damage and modification sites of DNA samples of different lengths and different sources, and is convenient to use. It is easy to operate, and has no special requirements on samples, high accuracy, low background impact and high resolution. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112745373-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112745373-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111690718-A |
| priorityDate | 2018-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 119.