http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109266723-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c377af5d5475608172f86d6d497bb3cd |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2018-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce3734633d9a495f9ddde8a4e2abda5f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_555d1818ffc11f4f756c08283ccf652d |
| publicationDate | 2019-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109266723-A |
| titleOfInvention | Rare mutation detection method, kit and application thereof |
| abstract | The invention discloses a rare mutation detecting method, a kit thereof and an application thereof, and the method is a U‐ARMS method. The detection method provided by the invention combines ARMS and locked nucleic acid (LNA) mutation enrichment and Taqman-MGB fluorescence detection technology, utilizes LNA to enhance mutant base recognition ability, and ARMS primers perform specific PCR amplification on mutant target sequence, Taqman ‐MGB probe detects the amplified product and identifies specific mutations based on fluorescent PCR. Compared with mutation detection techniques such as sanger sequencing, chip detection, and second-generation sequencing, the present invention has low cost for detecting mutations. Strong specificity, high sensitivity, simple and fast operation. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113308519-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110628883-A |
| priorityDate | 2018-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.