http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109266721-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate | 2018-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109266721-B |
| titleOfInvention | Method for detecting telomerase activity based on non-quenching molecular beacon |
| abstract | The invention belongs to the technical field of biological detection, and particularly relates to a method for detecting telomerase activity based on a non-quenching molecular beacon. The invention provides a 2-aminopurine quenching-free molecular beacon detection method for detecting telomerase activity in cancer cells. Telomerase is extracted from cancer cells, a catalytic repeat unit (TTAGGG) n is added to a substrate chain to generate an extension product of telomere repeat, a primer is extended along a template to form a double chain along with the addition of KF polymerase, the chain of 2-AP MB in the double chain can be degraded by T7exonuclease, a free 2-AP molecule and a primer extension chain are released, the free primer extension chain can be combined with other 2-AP MB to form a new double chain, and the next round of T7exonuclease degradation is triggered. Compared with the molecular signal method in the prior art, the invention realizes double signal amplification, has high sensitivity and specificity, and has important significance for the development of antitumor drugs. |
| priorityDate | 2018-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 105.