http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109266672-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_192f8f5667e2a5bf1638b57fef9165b2 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2018-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7edfd04bac1be36d9bd9e25c6931c49b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_025c16422e9d8210991cf76d6c76e411 |
| publicationDate | 2019-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109266672-A |
| titleOfInvention | Method for preparing T carrier |
| abstract | The invention discloses a preparation method of a T vector, which uses a thio-modified primer to amplify a plasmid vector; and uses a restriction endonuclease Dpn I to digest the template plasmid molecule to eliminate a negative background clone caused by a circular plasmid template; The PCR-amplified linearized vector was subjected to 5' exonuclease digestion treatment to obtain a T vector. In the above manner, the linearization fragment of the T vector can be prepared by direct and simple PCR amplification of the method of the present invention, and the technical defects of the restriction endonuclease method and the terminal deoxynucleotidyl transferase method are eliminated, and the simple treatment can be performed. The T vector was obtained. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110129347-A |
| priorityDate | 2018-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.