| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_98ff87faa2dbe4480c778d12da9bd42a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-00021 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate |
2018-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fec65dcdb0918bd0e6480127787ed5ab
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da5e838f1ba8b140cb547abcabd19660
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc8925cd5c7a94d55eb18b3021437957
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0cd603019ad619bc349ac8b323758d93
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aeb070a2625fcd53ae4f626419b51c7c |
| publicationDate |
2019-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-109266643-A |
| titleOfInvention |
Infectious clone of nematode virus in grape berry and construction method thereof |
| abstract |
The invention discloses a nematode infective clone of grape berry and a construction method thereof. The infective cloning vector is a seamlessly cloned fragmented grape berry endogenous necrosis virus genome fragment ligated into the pCB301‐2x35S-HDV RZ NOS vector containing the double 35S promoter and the ribozyme HDV-RZ. obtain. The vector was transferred to Agrobacterium EHA105 competent cells by freeze-thaw method, and the infectivity was verified by inoculating western tobacco and grapes with Agrobacterium. The GINV infective clone constructed by the invention can stably and efficiently express in the Agrobacterium EHA105 strain, and can successfully infect western tobacco and grape hosts. The acquisition of GINV invasive clones makes GINV studies easy to operate at the DNA level, providing a powerful tool for subsequent research on the biological properties and pathogenicity of GINV. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113215189-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113215189-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111500787-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111500787-B |
| priorityDate |
2018-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |