http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109266595-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y403-01019 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01009 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y102-01002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0016 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04 |
| filingDate | 2018-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109266595-B |
| titleOfInvention | Construction and application of recombinant bacterium for producing L-2-aminobutyric acid by converting L-threonine |
| abstract | The invention discloses construction and application of a recombinant bacterium for producing L-2-aminobutyric acid by converting L-threonine, belonging to the technical field of biological engineering. The production method provided by the invention utilizes a recombinant bacterium for expressing double plasmids, realizes efficient expression of three enzymes at the same time, converts L-threonine into L-2-aminobutyric acid, is coupled with a coenzyme regeneration system, and converts NAD + into NADH, so that the concentration of NADH in the system is relatively stable, and the conversion can be efficiently carried out. Furthermore, CO2 converted from ammonium formate in the process can be dissolved in ammonia water generated in a conversion system, so that the method has little environmental pollution and industrial application value. The method has the advantages of mild conversion conditions, strong specificity, low cost, short conversion time and the like. The method is adopted to prepare the L-2-aminobutyric acid, 40g/L L-threonine is added, the concentration of the obtained product L-2-aminobutyric acid is 43.3g/L, and the conversion rate is more than 99.9%. |
| priorityDate | 2018-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 499.