| abstract |
Method for preparing quinclorac hapten and antigen and application thereof, characterized in that said quinclorac hapten is brominated from quinclorac and (1,3-dioxanthylethyl) Magnesium reacts to form acetal quinclorac and is obtained by reacting with trifluoroacetic acid; the quinclorac antigen is obtained by coupling a quinclorac hapten with a carrier protein. The antigen prepared by the present invention exhibits a specific quinclorac acid antigenic determinant, making it possible to screen for a highly specific monoclonal antibody to quinclorac. The produced antibody has high specificity and high sensitivity, and can be used for establishing an enzyme-linked immunosorbent assay and a rapid determination method for colloidal gold test paper, thereby realizing rapid detection of quinclorac in food and tobacco soil. |