| abstract |
The invention discloses a preparation method of medical beauty grade placental base decidua stem cell inner fluid, in particular to the technical field of cell application. The preparation method is as follows: placenta selection, 35-40 week neonatal disc-shaped placenta and primary placenta are selected; placental base decidua is separated, the placenta is repeatedly washed with PBS buffer containing gentamicin, and the placental base is cut by ophthalmology The decidua part was placed in a petri dish; an ultrafiltration membrane was selected, and the placental basal decidua stem cell lysate was membrane-separated, stored frozen at ‑20°C, and thawed at room temperature for medical and cosmetic use. The method has the advantages of easy operation, low risk of immune reaction, high activity, obvious effect, etc., and effectively utilizes hospital medical waste, with low preparation cost and strong operability. Breaking through the skin absorption barrier, the medical beauty grade placental decidual stem cell inner fluid is deeply introduced into the subcutaneous tissue, and is quickly absorbed by the skin tissue to play a role, thereby producing a curative effect. |