| abstract |
The invention discloses a fluorescent quantitative PCR primer, probe, kit and method for detecting Cryptosporidium parvum, and relates to the technical field of molecular biology. The sequence of fluorescent quantitative PCR primers for detecting Cryptosporidium parvum of the present invention is SEQ ID NO. 1 and 2, and the sequence of fluorescent quantitative PCR probe is SEQ ID NO. 3; the reporter fluorescent group CY5 of the probe quenches fluorescence The group is BHQ1. The detection kit of the present invention includes: the above-mentioned primer pairs, probes, Tris-HCl buffer, KCl, MgCl 2 , dNTP, DMSO, glycerol, UDG enzyme and Taq enzyme; the kit can complete micro-cryptolysis within 100 minutes Sporozoite detection. The detection kit developed by the invention provides a new detection method for the pathogen of Cryptosporidium parvum, with simple operation, strong sensitivity and specificity. |