http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109234340-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-56 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-56 |
| filingDate | 2018-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109234340-B |
| titleOfInvention | Method for catalytically synthesizing rebaudioside M by recombinant yeast |
| abstract | The invention relates to a method for catalytically synthesizing rebaudioside M by recombinant yeast, belonging to the technical field of food additives. The technical problem solved by the invention is to provide a method for catalytically synthesizing rebaudioside M by using recombinant yeast with high conversion rate. The method comprises the steps of taking rebaudioside A as a substrate, taking recombinant yeast as a catalyst, catalyzing the substrate to react in the presence of sucrose, zinc chloride and trisodium citrate, filtering after the reaction, heating filtrate to inactivate recombinant microorganisms to obtain a crude rebaudioside M solution, and then purifying and concentrating to obtain rebaudioside M; the recombinant yeast contains an EUGT11 encoding gene and an UGT76G1 encoding gene, and the pgm gene, the glgC gene and the agp gene are knocked out from the recombinant yeast. The invention adopts specific recombinant yeast as a catalyst, realizes whole-cell catalytic conversion, has simple method, can obtain rebaudioside M by controlling the conversion conditions, and has the conversion rate of more than 90 percent. The method of the invention does not need to add UDP or UDPG, and has lower production cost. |
| priorityDate | 2018-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 89.