http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109234318-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-645 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y103-01 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-00 |
| filingDate | 2018-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109234318-B |
| titleOfInvention | Method for improving monascus extracellular pigment |
| abstract | The invention provides a method for improving monascus extracellular pigment, which is to knock out genes of sterol reductase ERG4/ERG24 family proteins in an ergosterol synthesis pathway coded in monascus. The invention knocks out the ERG4/ERG24 genes in monascus by an agrobacterium-mediated transformation technology. The invention starts from a plant binary plasmid pCAMBIA1300 vector, and transforms the recombinant plasmid into monascus by mediating by agrobacterium tumefaciens EHA105, thereby remarkably improving the production level of extracellular pigment, and the result shows that the recombinant strain has better passage stability. The liquid state fermentation result shows that the parent strain extracellular monascus pigment is 4.92U/mL, the recombinant strain extracellular monascus pigment constructed by the invention reaches 6.52U/mL, and the production amount of the extracellular pigment is improved by 32.52 percent, which indicates that the method can obviously improve the yield of the monascus extracellular pigment. |
| priorityDate | 2018-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.