http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109234310-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2018-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109234310-B |
| titleOfInvention | Recombinant vector for rapidly obtaining non-transgenic gene editing plants and method of use |
| abstract | A recombinant vector for rapidly obtaining a transgenic gene editing plant and a method for using the same, the recombinant vector is inserted into the original vector and contains PAP1 and NtFT expression elements, the expression elements are named PF Cas sete, and its nucleotide sequence is as SEQ ID No. 1; the original vector is a CRISPR/Cas9 vector for plant gene editing containing sgRNA gene, Cas9 gene and screening tag gene; the NtFT expression element produces a protein that promotes plant early flowering, and the PAP1 expression element produces a plant anthocyanin biosynthetic protein. The invention can greatly shorten the time for obtaining gene editing mutant progeny, and screen progeny plants by plant color, which not only retains the progeny of the target gene mutation, but also ensures that the mutant progeny does not contain transgenic fragments, and can significantly improve the gene Editing efficiency. The method of the invention is simple, fast, cheap and efficient. |
| priorityDate | 2018-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.