http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109234252-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-12 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 |
filingDate | 2018-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109234252-B |
titleOfInvention | Imprinted lipase and application thereof |
abstract | The invention discloses an imprinted lipase and application thereof, wherein the imprinted lipase is prepared by the following method: adding the imprinted molecules into lipase liquid, stirring for dissolving, adjusting the pH to 7.2-7.8, adding macroporous resin after stirring for imprinting reaction, filtering the reaction liquid after stirring for adsorption, washing and drying filter cakes to obtain imprinted lipase; according to the invention, maltose or sucrose is used as the imprinting molecule, compared with organic acid and ester compounds, the imprinting system does not need a surfactant and an alcohol cosolvent, and the imprinting molecule is better combined with lipase molecules in an aqueous solution. The maltose imprinted lipase has a wide application range in catalyzing oligosaccharide esterification, takes sucrose as an example to catalyze the synthesis of ethyl ester, the esterification rate and the esterification selectivity are both superior to those of non-imprinted lipase, the esterification rate of the maltose imprinted lipase is as high as 86.6%, and the esterification selectivity is as high as 85.4%. |
priorityDate | 2018-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.