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filingDate 2018-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2a91c2b4f302c5136a8812362f361e9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42d5c2ec9d543bd24ec3b93ab6f2092f
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publicationDate 2019-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109234252-A
titleOfInvention A kind of imprinted lipase and its application
abstract The invention discloses an imprinted lipase and an application thereof. The imprinted lipase is prepared by the following method: adding imprinted molecules into a lipase enzyme solution, stirring and dissolving, adjusting the pH to 7.2-7.8, and after stirring the imprinting reaction is complete, adding After the macroporous resin is stirred and adsorbed completely, the reaction solution is filtered, and the filter cake is washed and dried to obtain the imprinted lipase; the present invention uses maltose or sucrose as the imprinted molecule, and compared with organic acids and ester compounds as the imprinted molecules, the imprinted system does not. Surfactant and alcohol co-solvent are required, and the binding of imprinted molecules to lipase molecules is better in aqueous solution. Maltose-imprinted lipase has a wide range of applications in catalyzing the esterification of oligosaccharides. Taking sucrose as an example, it catalyzes the synthesis of its ethyl ester, and the esterification rate and esterification selectivity are better than those of unimprinted lipase. The esterification of the present invention The yield is as high as 86.6%, and the esterification selectivity is as high as 85.4%.
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priorityDate 2018-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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