http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109232813-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08J2333-26 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-28047 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08F220-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-268 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J13-0065 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J9-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F222-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F220-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
| filingDate | 2018-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109232813-B |
| titleOfInvention | Preparation method of HRP (horse radish peroxidase) imprinted hydrogel |
| abstract | The invention discloses a preparation method of HRP (horse radish peroxidase) imprinted hydrogel, which is characterized in that acrylamide (AAm) is used as a functional monomer, Methylene Bisacrylamide (MBAA) is used as a cross-linking agent, Ammonium Persulfate (APS) is used as a catalyst, Tetramethylethylenediamine (TEMED) is used as an initiator, and horseradish peroxidase (HRP) is used as a template molecule, HRP imprinted hydrogel with the thickness of 1mm is prepared by in-situ polymerization according to a certain proportion, the template molecule is washed off, a sample solution is directly dripped on the surface of the gel, unbound molecules are washed off by adsorption, and 3,3',5,5' -Tetramethylbenzidine (TMB) -H is adopted 2 O 2 The system develops color, and the generated color can be directly detected by naked eyes. The preparation method is simple and low in cost, and the prepared HRP imprinted hydrogel is convenient to detect and can be repeatedly used for many times, and can be used for identifying HRP in a serum sample with high selectivity, effectively removing matrix interference and having great application value and market prospect. |
| priorityDate | 2018-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.