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filingDate 2018-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3497b76f641f9c3a2b200fe6338e5e8
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publicationDate 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109212181-A
titleOfInvention A kind of neutrophil gelatinase-associated lipocalin detection kit and preparation method thereof and application method
abstract The invention discloses a kind of neutrophil gelatinase-associated lipocalin detection kit and preparation method thereof and application methods;It include magnetic particle suspension, NGAL calibration object and the luminous substrate solution of fluorescein-labeled NGAL antibody-solutions, the NGAL antibody-solutions of alkali phosphatase enzyme mark, coating anti-fluorescein antibody in the kit;Kit of the invention combines immune magnetic particle technology and chemiluminescence, it provides a kind of close to homogeneous reaction system, it allows the NGAL antibody of linkage flag tracer first to react with detection antibody with the NGAL antibody for connecting bridging object, forms the compound of double antigens sandwich structure;Due to during forming double antigens sandwich compound, it is all small-molecule substance with labelled antigen, its steric hindrance is far smaller than the steric hindrance of magnetic microsphere, and Ag-Ab association reaction can be promoted sufficiently to carry out, so that the detection performances such as sensitivity, precision, detection range greatly improve.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111337664-A
priorityDate 2018-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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