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inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ef0b4d26e68f2659084aa5355f85492
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publicationDate 2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109187818-A
titleOfInvention A method for identifying biomarkers of chronic obstructive pulmonary disease and detection kit thereof
abstract The present invention provides biomarkers and applications for identifying chronic obstructive pulmonary disease. The biomarkers are free glucose and mannose obtained from patient serum through high performance liquid chromatography (HPLC) derivatized with 1-phenyl-5-methylpyrazolone (PMP) before the column. The technical scheme of the invention has the advantages of simple pretreatment, short analysis time, reasonable price of the instrument, conforming to routine use, simple and easy-to-learn operation steps, high detection result accuracy, only need to collect blood for HPLC analysis to distinguish normal people from patients, and all the The amount of serum required is very small, and the blood collection volume is less than 1mL. The obtained results show that this analytical method can quickly analyze the free mannose and glucose in serum, and can calculate the concentration of mannose and glucose and their peak areas, which is useful for studying the relationship between serum free mannose and glucose and diseases, searching for The clinical detection of new chronic obstructive pulmonary disease markers is of great significance.
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