http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109182371-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_72de4ac6b66c3ef9bf8ed356540a7702 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H1-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
filingDate | 2018-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c952c937e74fa184df58b05488c225fe |
publicationDate | 2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109182371-A |
titleOfInvention | A genotype-independent approach to plant gene editing |
abstract | A genotype-independent plant gene editing method relates to the field of plant genetic engineering, and solves the problems that existing gene editing is limited by genotype and requires tissue culture to generate a large amount of genetic variation, which is not conducive to the elimination of transgenic components of offspring. . The invention uses transgenic plants containing gene editing target gRNA and gene editing system elements and plant lines with haploid induction efficiency as materials to prepare an intermediate vector with tool properties that can be used for gene editing; the intermediate vector is used as the male parent , induce plants to produce haploids and complete gene editing at the same time; after natural or artificial doubling, new plant varieties/lines homozygous for targeted gene editing are finally obtained. After preparing the gene editing intermediate vector tool, the method does not need to carry out complex genetic transformation and tissue culture process, and can realize the gene editing of different genotypes of the same plant only by the method of artificial cross-pollination. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116004705-A |
priorityDate | 2018-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.