http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109182256-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0653 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2018-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109182256-B |
| titleOfInvention | Separation culture and induction method of precursor adipocytes of nibea albiflora |
| abstract | The invention relates to the technical field of cell culture, and discloses a separation culture and induction method of precursor adipocytes of nibea albiflora, wherein the separation culture comprises the following steps: 1) firstly, digesting the fat tissue mass of the nibea albiflora by using a pancreatin solution, and then treating the fat tissue mass of the nibea albiflora by using a type II collagenase digestion solution; 2) and inoculating the dissociated precursor adipocytes of the nibea albiflora into a cell culture flask, and culturing by adopting a precursor adipocyte culture medium to form a cell monolayer. The induction method comprises digesting the obtained precursor adipocytes of the nibea albiflora with pancreatin solution, inoculating into a 6-well plate for culture, and transferring to a precursor cell induction culture medium for continuous culture. The method can successfully obtain the precursor adipocytes of the nibea albiflora and further induce the precursor adipocytes to obtain the mature adipocytes, has the advantages of simple and easy method, good repeatability, low cost and rapid cell growth, and can provide an effective in-vitro cell model for the molecular cell biology research of the nutrient metabolism of the nibea albiflora. |
| priorityDate | 2018-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 144.