http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109182233-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A62D2101-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A62D3-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D101-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D3-02 |
| filingDate | 2018-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109182233-B |
| titleOfInvention | Polycyclic aromatic hydrocarbon degrading engineering bacterium and engineering modification method and application thereof |
| abstract | The invention relates to a polycyclic aromatic hydrocarbon degrading engineering bacterium and an engineering modification method and application thereof. The gene catA2 which is a degradation gene for coding the cyclolytic dioxygenase is obtained by cloning pseudomonas aeruginosa SA1, and the nucleotide sequence is shown as SEQ ID No. 1; the nucleotide sequence of the specific oprL promoter is shown as SEQ ID No. 2; selecting a broad-host plasmid pBBR1MCS-5 as a vector; the catA2 gene and the oprL promoter are respectively cloned into a pBBR1MCS-5 vector, transferred into a receptor cell for cloning and amplification, and positive clones are screened to obtain a target recombinant vector pBBR1MCS-5-oprL-catA 2; the pseudomonas aeruginosa SA1 is used as a host, and the recombinant vector pBBR1MCS-5-oprL-catA2 is transformed into pseudomonas aeruginosa strains by an electric shock method to construct engineering pseudomonas aeruginosa. Has the capability of degrading polycyclic aromatic hydrocarbon. |
| priorityDate | 2018-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.