http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109161536-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-04001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1229 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-305 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01048 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 |
| filingDate | 2018-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109161536-B |
| titleOfInvention | Enzyme preparation for preparing uridylic acid and method for preparing uridylic acid by enzyme catalysis |
| abstract | The invention relates to an enzyme preparation for preparing uridylic acid and a method for preparing the uridylic acid by enzyme catalysis, wherein feed liquid containing uridine is used as a substrate, escherichia coli cell breaking liquid with uridine kinase and polyphosphate kinase activities, sodium hexametaphosphate, magnesium sulfate and a small amount of ATP are added, and the enzymatic reaction is carried out under the reaction conditions of pH8.0 and temperature 30 ℃ to synthesize the uridylic acid. In the coupled catalytic reaction system, uridine kinase is responsible for catalyzing uridine and ATP to produce uridylic acid, and ADP is formed along with ATP dephosphorylation. Polyphosphate kinase is responsible for catalyzing sodium hexametaphosphate and ADP to form ATP, so that ATP regeneration in the reaction is realized. The method for producing uridylic acid provided by the invention has the advantages of low raw material price, short period, simple and convenient operation, environmental protection and high yield, and has good industrial application value. |
| priorityDate | 2018-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 495.