http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109160947-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-795 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2018-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109160947-B |
| titleOfInvention | Preparation method and application of spirulina phycocyanin |
| abstract | The invention belongs to a separation and extraction technology of natural product active ingredients of light industry and chemical industry biotechnology in biology and new medicine, relates to the field of extraction and application, and particularly relates to an extraction method and application of spirulina phycocyanin. The preparation method comprises the following steps: 1) pulverizing dried Spirulina, and sieving; 2) adding phosphate buffer solution into the spirulina powder, and uniformly mixing; 3) swelling the feed liquid in an environment of 4 ℃, and performing superfine shearing on the feed liquid after swelling; 4) centrifuging the extracting solution to obtain a supernatant; 5) slowly adding ammonium sulfate into the supernatant, standing, and centrifuging to obtain supernatant; 6) continuously and slowly adding ammonium sulfate into the supernatant, standing, and centrifuging to obtain blue precipitate; 7) freeze drying the precipitate to obtain phycocyanin. The invention adopts the swelling-superfine shearing method combined with the wall breaking technology, the time consumption is short, the phycocyanin yield is high, and the prepared spirulina phycocyanin extract has obvious inhibition effect on the proliferation of HeLa cells. |
| priorityDate | 2018-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.