http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109136236-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 |
| filingDate | 2018-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109136236-B |
| titleOfInvention | A directed evolution approach to improve the specificity of lead-binding proteins |
| abstract | The invention discloses a directed evolution method for improving the specificity of lead-binding proteins. The steps are: (1) using error-prone PCR technology to amplify the lead-binding protein gene pbrR, and linking it into a screening plasmid containing a bidirectional screening system; (2) transforming The host cell E.coli DH5α was coated on a screening plate for directional screening; (3) The mutant protein M (PbrR‑C134R) obtained by screening was preliminarily evaluated. The invention applies the directional evolution technology to directionally transform the lead-binding protein with unknown three-dimensional structure, and further reduces the interference of zinc ions and improves the specificity of the lead-binding protein. Through targeted screening and preliminary evaluation of mutant proteins, the amino acid mutation sites related to the specificity of lead-binding proteins were analyzed and determined, which laid a foundation for the preliminary analysis of the relationship between lead-binding proteins and metal ion binding specificities. At the same time, it provides a novel and facile engineering strategy for directional engineering of the specificity of unknown metal regulatory proteins of the same family structure. |
| priorityDate | 2018-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.