http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109134594-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K5-0215 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
filingDate | 2017-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-109134594-B |
titleOfInvention | Method for preparing glutathione by enzyme method |
abstract | The invention discloses a method for preparing glutathione by an enzyme method, which comprises the following steps: (1) glutathione is generated in a reactor by using GshF enzyme, ATP regenerative enzyme and AK enzyme; (2) separating the immobilized GshF enzyme, ATP regenerating enzyme and AK enzyme in a reactor, or separating the free GshF enzyme using a filtration device; (3) separating the filtrate obtained in the step (2) to obtain a crude product GSH and a small amount of ATP, ADP and AMP; (4) and (4) concentrating, crystallizing and drying the GSH obtained in the step (3) to prepare the finished product GSH. The invention has the following advantages: adenosine is used for replacing ATP or AMP, so that a large amount of cost is saved for industrial production; reaction conditions for GSH production are optimized, and the reaction rate is high; a stable enzyme recovery system is established, and the method is energy-saving and environment-friendly; the by-product can be directly used for cyclic reaction or collected in a centralized way for producing ATP, and the operation is simple. |
priorityDate | 2017-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1266.