http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109097333-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P29-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P5-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P9-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0665 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P29-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P5-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P9-14 |
| filingDate | 2018-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109097333-B |
| titleOfInvention | It resists cell ageing and extends the mescenchymal stem cell and its preparation method and application of blood glucose-control effect time-histories |
| abstract | Present disclose provides a kind of methods for preparing and resisting the mescenchymal stem cell of cell ageing and extension blood glucose-control effect time-histories, and this method comprises the following steps: S1, will be mixed to form RNP compound for sgRNA the and Cas9 albumen for knocking in site of gene insertion safety island；S2, the AAV virion to be transfected for carrying GPX7 gene is formed；S3, the suspension of stem cell is mixed with the suspension of the RNP compound and carries out electricity turn；S4, transfection of the suspension of AAV virion to be transfected to carry out is added；S5, it carries out monoclonal culture and filters out to have carried out the monoclonal cell strain that gene orientation is knocked in；S6, amplification and Induction of committed differentiation become MSC.The disclosure additionally provides the mescenchymal stem cell and application thereof that the above method is prepared.Through the above technical solutions, present invention obtains a kind of resistance cell ageing and the multipotential stem cell preparation methods of extension blood glucose-control effect time-histories. |
| priorityDate | 2018-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 309.