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filingDate 2018-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109091602-B
titleOfInvention Effective component of semen allii tuberosi, extraction method and application thereof in preparing liver injury protection medicine
abstract The invention relates to a method for extracting effective components of semen allii tuberosi, which comprises the following steps: 1) performing flash extraction on the crushed semen allii tuberosi, the fried semen allii tuberosi or the salt-roasted semen allii tuberosi with 20-50% ethanol, and concentrating the extracting solution to obtain an extract; dispersing the extract in water, extracting with dichloromethane, adsorbing the water layer with macroporous adsorbent resin, sequentially gradient-eluting with water, 30% ethanol and 60% ethanol, and collecting 60% ethanol eluate; 2) and (3) performing rotary evaporation on the 60% ethanol eluent, dissolving the eluent with water, and purifying the eluent by an ODS open column, wherein the volume ratio of methanol to water is 1.8-2.5: 1, eluting, detecting by thin-layer chromatography, mixing the same components, and concentrating under reduced pressure. Experimental research shows that: the effective components of semen Allii Tuberosi can antagonize mouse liver tissue injury caused by carbon tetrachloride, and has certain protective effect.
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