| abstract |
The invention discloses a kind of human chorionic gonadotrophin detection kit and preparation method thereof and application methods；The kit includes calibration object, quality-control product, anti-reagent A, anti-reagent B, magnetic particle reagent and luminous substrate；Calibration object and quality-control product are to use protein-contg buffer solution Total- β hCG antigen, and be diluted to prepare in the buffer containing newborn bovine serum and obtain；Anti- reagent A is the Total- β hCG coated antibody of marked by fluorescein isothiocyanate, and anti-reagent B is the Total- β hCG labelled antibody of alkali phosphatase enzyme mark；Magnetic particle reagent is made by fluorescein isothiocynate antibody and magnetic particle coupling.The invention further relates to the methods of human chorionic gonadotrophin (Total- β hCG) content in the preparation method of the kit and application kit quantification detection human serum.Kit reliable performance, high sensitivity, the range of linearity are wide, and semi-automatic, full-automatic instrument can be cooperated to use. |