http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109060782-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-76 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 |
| filingDate | 2018-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109060782-B |
| titleOfInvention | Preparation method and application of luminous detection reagent for early pregnancy of cat |
| abstract | The invention provides a preparation method of a luminous detection reagent for early pregnancy of cats, which comprises the steps of carrying out centrifugal concentration on a relaxin antibody R021 and acridinium ester to remove impurities, and carrying out reaction according to the weight ratio of 3: the method comprises the following steps of fully and uniformly mixing relaxin antibody R021 and acridinium ester in a mass ratio of 1, carrying out labeling coupling, slightly shaking for labeling for 3 hours at room temperature under the condition of pH 6.3, dialyzing with 0.1M PBS, removing unbound acridinium ester, purifying by a Sephadex G50 gel chromatography column, collecting a product with high luminous intensity on a chemiluminescence apparatus, combining the purified relaxin antibody R021 and acridinium ester conjugate, storing in a luminous reagent buffer solution, subpackaging, and storing for later use. Whether the pregnant woman is pregnant can be detected 14 days after mating, the detection is greatly earlier than the detection of an external observation method, a palpation method and ultrasonic waves for 30 days, and the early pregnancy detection is really realized. |
| priorityDate | 2018-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.