| abstract |
The present invention provide it is a kind of extract ganoderma lucidum total DNA reagent and its application, from its mycelium of 50 mg or so and fructification sample, adsorb, rinse by broken cracking, DNA, eluting and etc., extraction total DNA rapidly and efficiently, for fresh dry-eye disease, the total DNA for being suitable for the molecular biology experiments such as ITS identification equally also can extract.Further it is demonstrated experimentally that this method can also be applied on the edible and medical fungis such as Cordceps militaris, Agricus blazei, grifola frondosus.This method single sample extraction time foreshortens to 15 min, and extracted DNA integrality is good, with high purity, can be directly used for the test of the downstream molecular biologies such as PCR, Real-Time PCR, molecular labeling. |