http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109055311-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2531-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2018-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109055311-B |
| titleOfInvention | Human lymphocyte culture method based on serum-free culture medium |
| abstract | The invention discloses a human lymphocyte culture method based on a serum-free culture medium, which comprises the following steps: adding human peripheral blood mononuclear cells into an RPMI1640 culture medium to prepare a cell suspension, and after incubating for 1-3 hours at room temperature, separating out human T lymphocytes positive to CD 8; adding selected CD8 positive human T lymphocyte into serum-free culture medium to prepare cell suspension, culturing for 1-2 days, replacing with fresh serum-free culture medium, adding pMHC-loaded nanoparticle, adding the serum-free culture medium, and adjusting cell concentration of peripheral blood mononuclear cell to (0.5-1). times.10 6 And (4) inoculating per mL to obtain the antigen specific T lymphocyte. The culture method provided by the invention can realize the culture and efficient amplification of the human T lymphocyte, and the human T lymphocyte has enhanced capacity of specifically killing the tumor cells targeted by the antigen. |
| priorityDate | 2018-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 281.